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Squamous intraepithelial lesion is an abnormal growth of epithelial cells on the surface of the cervix that may lead to cervical cancer. Analytical protocols for the determination of squamous intraepithelial lesions are in high demand, since cervical cancer is the fourth most diagnosed cancer among women in the world. Here, paper spray ionization mass spectrometry PSI-MS is used to distinguish between healthy negative for intraepithelial lesion or malignancy and diseased high-grade squamous intraepithelial lesion blood plasmas.

A total of 86 blood samples of different women 49 healthy samples, 37 diseased samples were collected, and the plasmas were prepared. No additional step of sample preparation was necessary. The interval-successive projection algorithm linear discriminant analysis iSPA-LDA was applied to the PSI mass spectra, showing six ions mostly phospholipids that were predictive of healthy and diseased plasmas. This study provides evidence that PSI-MS may potentially be used as a fast and simple analytical technique for the early diagnosis of cervical cancer.

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